Born in 1970 in Prague, Ondřej Roskovec studied bassoon at the Prague Conservatoire with Jiří Formáček and at the Prague Academy of Performing Arts with professors Jiří Seidl and František Herman.
While still a student, he won awards at various competitions (e.g. Brno 1987 – 1st prize, Kraslice 1988 – 1st prize), becoming a laureate of the Prague Spring International Competition and receiving the award of the Swiss foundation Pro Harmonia Mundi in 1996. Between 1989 and 1993 Roskovec was a member of the Czech Nonet.
In 1993 he joined the Czech Philharmonic, becoming its solo bassoonist in 2000. In 1995 Roskovec was one of the founders of the Afflatus Quintet, a chamber ensemble that won a 1st prize at Munich's prestigious ARD International Music Competition in 1997. With the Afflatus Quintet, he has performed at many important venues in the Czech Republic, Europe and Japan. The quintet has recorded eight CDs, mostly with the Japanese company Octavia Records. Octavia also released two CDs with Roskovec's solo performances – “Combination” in 2006 and Bach Suites BWV 1007–9 in 2013.
Since 2002 Ondřej Roskovec has been teaching future bassoonists at the Prague Conservatoire. He is also one of the founders of the Summer Bassoon Academy in Rataje nad Sázavou.